As a result, a full kinetic read out of the cellular behaviour is obtained- objective and quantitative. Hence, the effect of signalling proteins, released by indirectly co-cultured effector cells, can easily be monitored in real time using xCELLigence RTCA. Have a look at our overview of all events. RTCA Plate Stations* can be placed inside standard cell culture incuba tors (RTCA MP Plate Station is shown), creating a temperature-, CO2-, and humidity-c ontrolle d environment througho ut an experiment. Xcelligence Rtca Mp Instrument, supplied by ACEA Biosciences, used in various techniques. Note the correlation between impedance values (Cell Index) and the number of adherent cells visible in the microscope. Compound-induced cytotoxicity of Hela cells. As cells adhere to and proliferate on the electrodes current flow is impeded, providing an extremely sensitive readout of cell number, cell size/morphology, and cell-substrate attachment quality. Neither the electrodes nor the cells are drawn to scale (they have been enlarged for clarity). Online product catalogs, news, articles, events, publications & more. No. This noninvasive measurement allows detection of changes in adherence, morphology, and viability without … If you continue using this website, we‘ll assume that you are happy to receive all cookies. Environmental XPRT is a global environmental industry marketplace and information resource. When cells reached confluence, the virus was added as indicated by the black vertical line. (C) Zoomed in brightfield image of shadowed electrodes and unstained human cells. This might be explained by irradiation-induced DNA damage that could be repealed by cellular DNA repair mechanisms. The xCELLigence RTCA SP and DP systems are available in the Center for Systems Biomedicine. (D) Real-time impedance traces for A549 cells exposed to drugs displaying a variety of mechanisms of action. This high aquisition rate in the range of milliseconds enables the detection of morphological changes that are due to cardiomyocyte contractility. Remarkably, upon addition of non-adherent effector cells to a confluent layer of target cells, Cell Index values will decline due to the cytolytic activity of the effector cells. Microelectrodes, integrated into the bottom of each well of the tissue culture E-Plates, detect minor changes of cellular impedance that occur upon changes in cell number, cell morphology, cell surface attachment, etc. This arrangement enables populations of cells to be monitored simultaneously and thereby provides exquisite sensitivity to: the number of cells attached to the plate, the size/morphology of the cells, and the cell-substrate attachment quality. Impedance electrodes on ACEA’s E-Plates. Download RTCA DP Brochure, RTCA SP (Single Plate) B) Impeded electron flow from one biosensor to the other due to cells attached/growing on gold microelectrodes, Figure 3: Schematic representation of the Cell Index (CI) value in the course of an apoptosis, I would like to be informed in future about trends, webinars and offers from OLS by email (*), I agree that my data will be used to process my request, High Content Screening Workstation WiScan® Hermes, Real-Time Live-Cell Imaging System xCELLigence® RTCA eSight, Real-Time Cell Analysis and Monitoring of Virus-Mediated Cytopathic Effects, xCELLigence RTCA MP, Real Time Cell Analyzer, xCELLigence RTCA SP, Real Time Cell Analyzer, xCELLigence RTCA HT, Real Time Cell Analyzer, xCELLigence RTCA DP, Real Time Cell Analyzer, xCELLigence RTCA S16, Real Time Cell Analyzer, xCELLigence RTCA Cardio, Real Time Cell Analyzer, xCELLigence RTCA Cardio ECR, Real Time Cell Analyzer, xCELLigence RTCA ePacer, Real Time Cell Analyzer, Continuously monitor live cell proliferation, morphology and viability, Different throughputs from 16 to 4×384 well formats, Kinetic read-out – capture both short (sec) and long-term effects (days), Transverse section of a single well of a CIM plate: a) Upper compartment with cells of interest; b) Microporous membrane with gold microelectrodes underside; c) Lower compartment containing chemoattractant, Cell, migrating through a pore of the CIM plate membrane. The xCELLigence RTCA system. 5.000 DU145 cells were seeded into each well of an E-Plate 96 and monitored every 15 minutes using a xCELLigence MP system. D) Unstained human cells, growing on microelectrodes, Figure 2: Schematic image of electron flow in a single well of an E-Plate. While this generic example involves drug addition when cells are confluent, impedance-based assays are extremely flexible and can also evaluate the rate and extent of initial cell adhesion to the electrodes, or the rate and extent of cell proliferation. While Cell Index values of non-treated control cells increased due to unhindered cell proliferation (black curve), decreasing Cell Index values of treated cells indicated cell death, morphological changes and cell detachment. B) Virus-induced CPE, indicated by changes in cellular morphology, cell surface attachment, cell-cell adhesion and decreased number of living cells, lead to a decrease of electrical impedance, measured by gold biosensors in real time. xCELLigence Technology is based on impedance measurement. Directly after addition of NK92 cells, the Cell Index values slightly decreased due to temperature changes within the medium. Ordering Informat ion for xCELLigence RTCA DP System Product Cat. Terms (B) Photograph of a single well in a 96-well E-Plate. Nevertheless, pre-incubation of the target cells with anti-IGF-1R led to enhanced cytolytic activiy, which strongly correlated with the amount of anti-IGF-1R. Data from App. Overview of cellular impedance apparatus. Real-time monitoring using xCELLigence RTCA revealed increased proliferation of T47D cells upon indirect co-culture with H295R cells. The Agilent xCELLigence real-time cell analysis (RTCA) dual purpose (DP) system provides a unique and powerful means to monitor cells in real time without the potential artifacts generated by using labels. Privacy, Our sites: With one single 16-well plate the S16 is the smallest member of the xCELLigence® RTCA family. Figure 2. WEBINAR – xCELLigence® RTCA CardioECR System: Preclinical Safety Assessment, iPSC-cardiomyocytes Functional Maturation and Assessment of Inotropic Compounds About this Webinar Cardiovascular (CV) toxicity is a leading cause of drug attrition and withdrawal. This gives you a maximal screening capacity of 1536 wells in total. The xCELLigence® RTCA MP system accommodates up to six 96-well microtiter E-Plates. Toxicity was quantified by calculating the inhibitory concentration (IC) from the Cell Index values of the dose-response curve 48 hours after irradiation. Therefore, xCELLigence Cardio ERC system is of relevance for any kind of in vitro assays, investigating drug induced cardic liability. A) Electron flow from one biosensor to the other through culture media only. xCELLigence system Real time, label free, cells analysis Riccardo Pasculli Field Application Specialist GSM: +420 731 127 717 E-mail: pasculli@accela.eu . xCELLigence RTCA MP Instrument The RTCA MP (multiple-plate) Instrument (Figure 2) also consists of a RTCA Analyzer and a RTCA Control Unit, but includes a RTCA MP Station designed for the flexible use of up to six E-Plates 96 in parallel, and includes the 2.0 version of the RTCA … No need for time-consuming manual detachment, staining and counting of cells from lower sides of membranes. Use xCELLigence Immunotherapy Kits with your real-time cell analysis (RTCA®) system for a non-invasive solution to a broad range of liquid cancer immunotherapies and suspension tumor cell killing … Irradiation-induced cytotoxicity of HEK-293 cells: Data from Application Note „Irradiation-Induced Cytotoxicity“. C) Zoomed-in photograph of gold microeletrodes on the E-Plate® well bottom. Antibody-dependent cellular cytotoxicity is a mechanism of cell-mediated immune defense that involves active lysis of target cells, mediated by effector cells from the immune system, like natural killer (NK) cells. Cell migration assays using the xCELLigence DP system have proven indispensable for our work, as it allows us to generate valuable, easily quantifiable kinetic data, combined with a simple and time-saving setup procedure. Sales, info@ols-bio.de To analyze cell-mediated cytolytic activity, the prostate cancer cell line DU145 was used as target cells, seeded into an E-Plate 96 and monitored every 15 minutes using xCELLigence MP system. To investigate irradiation-induced cell damage, HEK-293 cells were plated into a E-Plate 96 and treated with different doses of γ-irradiation (1 – 50 Gy). Currently there are no upcoming events. These plates can be operated simultaneously or independently to maximize productivity and save valuable laboratory time for multiple users. xCELLigence® Real Time Cell Analyzer (RTCA) allows the analysis of your cell culture in real time. The xCELLigence System from ACEA Biosciences is a microelectronic biosensor system for cell -based assays, providing dynamic, real-time, label-free cellular analysis for a variety of research applications in drug development, toxicology, cancer, medical microbiology,and virology.The xCELLigence system … Migration and Invasion: Besides the „normal“ E-Plates®, the DP system can be operated with CIM (Cell Invasion and Migration) Plates® to investigate migration and invasion of cells. I agree that my data will be used to process my request. I can revoke this consent at any time. Regarding the reduction in the overall workload and the consumption of reagents (i.e., e-plate 16 or 96-wells with the high throughput MP xCELLigence system) instead of 6-well plates), the RTCA assay is less … Similar to the other RTCA xCELLigence® members, the Cardio system is based on noninvasive measurement of electrical imedance to monitor changes in cellular morphology, proliferation and attachment. 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Cytotoxic compounds and analyzed every 15 minutes with a RTCA xCELLigence technology benefits from different adhesion properties of cells the. Microtiter E-Plates proliferation rate, cell size/shape, and the cellular behavior it arises from, is explained the. Cardioecr System seeding, cells start to die and subsequently detach from the cell Index values were to! Evaluate complete kinetics of cell-mediated cytolysis in a 96 microtiter plate nicely represented by a phase where cells exponentially...